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the bluescript sk1 vector  (Agilent technologies)


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    Structured Review

    Agilent technologies the bluescript sk1 vector
    The Bluescript Sk1 Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bluescript+sk1-vector/pm10828066-57-32-35
    Average 90 stars, based on 1 article reviews
    the bluescript sk1 vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Binding of Ca2+ and Zn2+ to Human Nuclear S100A2 and Mutant Proteins
    Article Snippet: Construction of Wild Type and Mutated Expression Vectors—To replace one or both of the functional Ca21 N- or C-terminal domain) of wtS100A2 (GenBankTM/EMBL accession number M87068) by a canonical loop (EF-domain) of human a-PV, the human cDNA (GenBankTM/ EMBL accession number X63070) was amplified by PCR on PerkinElmer and Hybaid Omni-Gene thermal cyclers, using specific oligonucleotides spanning the sequence of the EF-site of PV and specific restriction sites for subsequent cloning into the Bluescript SK1-vector (Stratagene).

    Functional Assay:

    Article Title: Binding of Ca2+ and Zn2+ to Human Nuclear S100A2 and Mutant Proteins
    Article Snippet: Construction of Wild Type and Mutated Expression Vectors—To replace one or both of the functional Ca21 N- or C-terminal domain) of wtS100A2 (GenBankTM/EMBL accession number M87068) by a canonical loop (EF-domain) of human a-PV, the human cDNA (GenBankTM/ EMBL accession number X63070) was amplified by PCR on PerkinElmer and Hybaid Omni-Gene thermal cyclers, using specific oligonucleotides spanning the sequence of the EF-site of PV and specific restriction sites for subsequent cloning into the Bluescript SK1-vector (Stratagene).

    Amplification:

    Article Title: Binding of Ca2+ and Zn2+ to Human Nuclear S100A2 and Mutant Proteins
    Article Snippet: Construction of Wild Type and Mutated Expression Vectors—To replace one or both of the functional Ca21 N- or C-terminal domain) of wtS100A2 (GenBankTM/EMBL accession number M87068) by a canonical loop (EF-domain) of human a-PV, the human cDNA (GenBankTM/ EMBL accession number X63070) was amplified by PCR on PerkinElmer and Hybaid Omni-Gene thermal cyclers, using specific oligonucleotides spanning the sequence of the EF-site of PV and specific restriction sites for subsequent cloning into the Bluescript SK1-vector (Stratagene).

    Polymerase Chain Reaction:

    Article Title: Binding of Ca2+ and Zn2+ to Human Nuclear S100A2 and Mutant Proteins
    Article Snippet: Construction of Wild Type and Mutated Expression Vectors—To replace one or both of the functional Ca21 N- or C-terminal domain) of wtS100A2 (GenBankTM/EMBL accession number M87068) by a canonical loop (EF-domain) of human a-PV, the human cDNA (GenBankTM/ EMBL accession number X63070) was amplified by PCR on PerkinElmer and Hybaid Omni-Gene thermal cyclers, using specific oligonucleotides spanning the sequence of the EF-site of PV and specific restriction sites for subsequent cloning into the Bluescript SK1-vector (Stratagene).

    Sequencing:

    Article Title: Binding of Ca2+ and Zn2+ to Human Nuclear S100A2 and Mutant Proteins
    Article Snippet: Construction of Wild Type and Mutated Expression Vectors—To replace one or both of the functional Ca21 N- or C-terminal domain) of wtS100A2 (GenBankTM/EMBL accession number M87068) by a canonical loop (EF-domain) of human a-PV, the human cDNA (GenBankTM/ EMBL accession number X63070) was amplified by PCR on PerkinElmer and Hybaid Omni-Gene thermal cyclers, using specific oligonucleotides spanning the sequence of the EF-site of PV and specific restriction sites for subsequent cloning into the Bluescript SK1-vector (Stratagene).

    Clone Assay:

    Article Title: Binding of Ca2+ and Zn2+ to Human Nuclear S100A2 and Mutant Proteins
    Article Snippet: Construction of Wild Type and Mutated Expression Vectors—To replace one or both of the functional Ca21 N- or C-terminal domain) of wtS100A2 (GenBankTM/EMBL accession number M87068) by a canonical loop (EF-domain) of human a-PV, the human cDNA (GenBankTM/ EMBL accession number X63070) was amplified by PCR on PerkinElmer and Hybaid Omni-Gene thermal cyclers, using specific oligonucleotides spanning the sequence of the EF-site of PV and specific restriction sites for subsequent cloning into the Bluescript SK1-vector (Stratagene).



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